| 1. | Chinensis were also studied . the developmental status of seedlings , vacuum infiltration time , type of agrobacterium tumefaciens used , all influence the transformation frequency
结果表明植株生长阶段和真空渗入处理时间，所用菌株类型均对转化率有影响。 |
| 2. | Negative pressure disposition in the process of agrobacterium tumefaciens infecting callus was found to be able to improve transformation frequency of indica tested rice apparently
以yeb培养基作为共培养培养基，对于农杆菌eha05 … ub m ）来说，菌液浓度为od值一0 |
| 3. | The transformation frequency achieved lo ^ cells / ug dna when pseudom onas fluorescent , was grown to 0 . 55 at odr , oo , and incubated for 2 minutes at 42 xh prior to treatment with 25mmol / l cacl2
获得克隆子lzng -一20a和lzr19一20a .在iptg诱导下，不管插人的尸2叹基因是正向还是反向，都能保护cyt人蛋白对大肠杆菌的致死作用 |
| 4. | Ph value increase from 4 . 5 to 6 . 5 strongly stimulated hairy root formation and higher ph value ( above 7 . 5 ) inhibited it . under the optimized condition , the transformation frequency of torenia approached 90 %
预培养和共培养培养基ph从4 . 5提高到6 . 5时，毛状根诱导率显著提高，但是当提高到7 . 5时，诱根率却显著下降。 |
| 5. | Using calli as explants , the transformation frequency could be greatly improved . using low ph medium ms to activate agrobacterium , and when its concentration is about od0 . 3 ~ 0 . 7 , are the best conditions for inoculation
Zing l活化农杆菌可提高转化效率； as可显著提高转化效率，其浓度以20t时效果最好；菌液浓度对转化效率也有影响，以od 。 |
| 6. | The transformation frequencies of exogenous plasmids in bmb171 were 7 . 5 103 - 8 . 1 108 folds as much as those in ybt - 1463 , and stability of exogenous introduced in 8mb 171 was obviously higher than in ybt - 1463
用pht3101等5种供体质粒电转化bmb171的效率比电转化ybt - 1463的效率显著提高，而转化导入的3种外源质粒在bmb171中的稳定性也明显高于出发菌株ybt - 1463 。 |
| 7. | The result of study on the factors affecting the transformation frequency showed : the leaf disc was pre - cultured for 2 days , infected for 20 minutes , co - cultivated with agrobacterium eha105 for 2 days , which could improve regeneration efficiency . the highest regeneration efficiency was 13 . 3 % in this experiment
实验结果表明：叶片预培养2d ，农杆菌浸染20min ，与农杆菌共培养2d时，筛选培养基上洋桔梗叶盘不定芽分化频率最高，达13 . 3 。 |
| 8. | Pcr , pcr - southern blot analysis , southern dot blot analysis of lettuce dna confirmed that adw gene had been integrated into the plant genome . the results also showed that the transformation frequency of pb - adw was higher than that of pbg - adw , which suggested that camv35s promoter would be better than pi ii promoter in the case of transgenic lettuce
( 3 )细胞核载体pb - adw 、 pbg - adw均采用农杆菌介导法将adw导入莴苣，细胞核转化获得了生长良好的抗性莴苣植株，经pcr 、 pcr - southern 、 southern斑点杂交分析证实， adw基因已整合到莴苣基因组中。 |
| 9. | Acetosyringone ( as ) could dramatically improve transformation frequency , its suitable concentration is 20 mg . l " 1 . before inoculated with agrobacterium , calli should be precultured 1 ? days , and the appropriate inoculation time is 4 ~ 8 minutes , and then cocultured for 3 ~ 4days . then the cocultured calli were transferred to ms medium containing 400 mg . l " 1 cef
7为宜；侵染前预培养1 2天，可显著提高转化效率；侵染时间以4 8分钟为宜；共培养时间3 4天较适合；转化后暗培养对转化效率没有影响；选择方法以延迟选择15天选择转化率最高。 |
